Local injection of stem cell factor (SCF) improves myocardial homing of systemically delivered c-kit + bone marrow-derived stem cells.
Identifieur interne : 002254 ( Main/Exploration ); précédent : 002253; suivant : 002255Local injection of stem cell factor (SCF) improves myocardial homing of systemically delivered c-kit + bone marrow-derived stem cells.
Auteurs : RBID : pubmed:18006465English descriptors
- KwdEn :
- Animals, Cell Movement (drug effects), Cell Separation, Hematopoietic Stem Cell Transplantation, Injections, Male, Mice, Mice, Inbred C57BL, Myocardial Infarction (physiopathology), Myocardial Infarction (therapy), Proto-Oncogene Proteins c-kit (analysis), Stem Cell Factor (administration & dosage).
- MESH :
- chemical , administration & dosage : Stem Cell Factor.
- chemical , analysis : Proto-Oncogene Proteins c-kit.
- drug effects : Cell Movement.
- physiopathology : Myocardial Infarction.
- therapy : Myocardial Infarction.
- Animals, Cell Separation, Hematopoietic Stem Cell Transplantation, Injections, Male, Mice, Mice, Inbred C57BL.
Abstract
Recent studies have shown that stem cell therapy may alleviate the detrimental effects of myocardial infarction. Yet, most of these reports observed only modest effects on cardiac function, suggesting that there still is need for improvement before widespread clinical use. One potential approach would be to increase migration of stem cells to the heart. We therefore tested whether local administration of stem cell factor (SCF) improves myocardial homing of intravenously infused lin-/c-kit+ stem cells after myocardial infarction.
DOI: 10.1093/cvr/cvm027
PubMed: 18006465
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Local injection of stem cell factor (SCF) improves myocardial homing of systemically delivered c-kit + bone marrow-derived stem cells.</title><author><name sortKey="Lutz, Matthias" uniqKey="Lutz M">Matthias Lutz</name><affiliation wicri:level="3"><nlm:affiliation>Department of Cardiology, Internal Medicine III, University of Heidelberg, INF 410, D-69120 Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Cardiology, Internal Medicine III, University of Heidelberg, INF 410, D-69120 Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Rosenberg, Mark" uniqKey="Rosenberg M">Mark Rosenberg</name></author><author><name sortKey="Kiessling, Fabian" uniqKey="Kiessling F">Fabian Kiessling</name></author><author><name sortKey="Eckstein, Volker" uniqKey="Eckstein V">Volker Eckstein</name></author><author><name sortKey="Heger, Thomas" uniqKey="Heger T">Thomas Heger</name></author><author><name sortKey="Krebs, Jutta" uniqKey="Krebs J">Jutta Krebs</name></author><author><name sortKey="Ho, Anthony D" uniqKey="Ho A">Anthony D Ho</name></author><author><name sortKey="Katus, Hugo A" uniqKey="Katus H">Hugo A Katus</name></author><author><name sortKey="Frey, Norbert" uniqKey="Frey N">Norbert Frey</name></author></titleStmt><publicationStmt><date when="2008">2008</date><idno type="doi">10.1093/cvr/cvm027</idno><idno type="RBID">pubmed:18006465</idno><idno type="pmid">18006465</idno><idno type="wicri:Area/Main/Corpus">002594</idno><idno type="wicri:Area/Main/Curation">002594</idno><idno type="wicri:Area/Main/Exploration">002254</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Movement (drug effects)</term><term>Cell Separation</term><term>Hematopoietic Stem Cell Transplantation</term><term>Injections</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Myocardial Infarction (physiopathology)</term><term>Myocardial Infarction (therapy)</term><term>Proto-Oncogene Proteins c-kit (analysis)</term><term>Stem Cell Factor (administration & dosage)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Stem Cell Factor</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Proto-Oncogene Proteins c-kit</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Movement</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Myocardial Infarction</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Myocardial Infarction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Separation</term><term>Hematopoietic Stem Cell Transplantation</term><term>Injections</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recent studies have shown that stem cell therapy may alleviate the detrimental effects of myocardial infarction. Yet, most of these reports observed only modest effects on cardiac function, suggesting that there still is need for improvement before widespread clinical use. One potential approach would be to increase migration of stem cells to the heart. We therefore tested whether local administration of stem cell factor (SCF) improves myocardial homing of intravenously infused lin-/c-kit+ stem cells after myocardial infarction.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">18006465</PMID><DateCreated><Year>2008</Year><Month>01</Month><Day>03</Day></DateCreated><DateCompleted><Year>2008</Year><Month>09</Month><Day>02</Day></DateCompleted><DateRevised><Year>2009</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0008-6363</ISSN><JournalIssue CitedMedium="Print"><Volume>77</Volume><Issue>1</Issue><PubDate><Year>2008</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Cardiovascular research</Title><ISOAbbreviation>Cardiovasc. Res.</ISOAbbreviation></Journal><ArticleTitle>Local injection of stem cell factor (SCF) improves myocardial homing of systemically delivered c-kit + bone marrow-derived stem cells.</ArticleTitle><Pagination><MedlinePgn>143-50</MedlinePgn></Pagination><Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">Recent studies have shown that stem cell therapy may alleviate the detrimental effects of myocardial infarction. Yet, most of these reports observed only modest effects on cardiac function, suggesting that there still is need for improvement before widespread clinical use. One potential approach would be to increase migration of stem cells to the heart. We therefore tested whether local administration of stem cell factor (SCF) improves myocardial homing of intravenously infused lin-/c-kit+ stem cells after myocardial infarction.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">Myocardial infarction was induced in mice via ligation of the left anterior descending artery and 2.5 microg of SCF were injected into the peri-infarct zone. Sham-operated mice and animals with intramyocardial injection of phosphate-buffered saline (PBS) served as controls. Twenty-four hours after myocardial infarction, lin-/c-kit+ stem cells were separated from murine bone marrow by magnetic cell sorting, labelled with the green fluorescent cell tracker CFDA or 111 Indium, and subsequently 750 000 labelled cells were systemically infused via the tail vein. Another 24 or 72 h later, respectively (i.e. 48 and 96 h after myocardial infarction), hearts were removed and analysed for myocardial homing of stem cells. Green fluorescent stem cells were exclusively detected in the peri-infarct zone of animals having prior SCF treatment. Radioactive measurements revealed that an intramyocardial SCF injection significantly amplified myocardial homing of lin-/c-kit+ stem cells compared to animals with PBS injections (3.58 +/- 0.53 vs. 2.28 +/- 0.23 cpm/mg/10(6)cpm, +60%, P < 0.05) and sham-operated mice without myocardial infarction (3.58 +/- 0.53 vs. 1.95 +/- 0.22 cpm/mg/10(6)cpm, +85%, P < 0.01). Similar results were obtained 72 h after stem cell injection.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">We demonstrate that intramyocardial administration of SCF sustainably directs more lin-/c-kit+ stem cells to the heart. Future studies will have to show whether higher levels of myocardial SCF (i.e. by virus-mediated gene transfer) can further improve homing of systemically delivered c-kit+ stem cells and thus favourably influence cardiac remodelling following myocardial infarction.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lutz</LastName><ForeName>Matthias</ForeName><Initials>M</Initials><Affiliation>Department of Cardiology, Internal Medicine III, University of Heidelberg, INF 410, D-69120 Heidelberg, Germany.</Affiliation></Author><Author ValidYN="Y"><LastName>Rosenberg</LastName><ForeName>Mark</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Kiessling</LastName><ForeName>Fabian</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Eckstein</LastName><ForeName>Volker</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Heger</LastName><ForeName>Thomas</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Krebs</LastName><ForeName>Jutta</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ho</LastName><ForeName>Anthony D</ForeName><Initials>AD</Initials></Author><Author ValidYN="Y"><LastName>Katus</LastName><ForeName>Hugo A</ForeName><Initials>HA</Initials></Author><Author ValidYN="Y"><LastName>Frey</LastName><ForeName>Norbert</ForeName><Initials>N</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>09</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Cardiovasc Res</MedlineTA><NlmUniqueID>0077427</NlmUniqueID><ISSNLinking>0008-6363</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Stem Cell Factor</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.10.1</RegistryNumber><NameOfSubstance>Proto-Oncogene Proteins c-kit</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Movement</DescriptorName><QualifierName MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Separation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Hematopoietic Stem Cell Transplantation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Injections</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Myocardial Infarction</DescriptorName><QualifierName MajorTopicYN="N">physiopathology</QualifierName><QualifierName MajorTopicYN="Y">therapy</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Proto-Oncogene Proteins c-kit</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Stem Cell Factor</DescriptorName><QualifierName MajorTopicYN="Y">administration & dosage</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2007</Year><Month>9</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2007</Year><Month>10</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>11</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>9</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>11</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">cvm027</ArticleId><ArticleId IdType="doi">10.1093/cvr/cvm027</ArticleId><ArticleId IdType="pubmed">18006465</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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